Yuh-Pyng Sher has completed her Ph.D. in cancer research from National Taiwan University at 2006. Supporting by 2007 NHRI Postdoctoral Fellowship Award, she had postdoctoral studies from Center for Molecular Medicine in China Medical University Hospital. Now, she is an associate professor in China Medical University and a deputy director in Research center for Chinese Herbal Medicine in China Medical University. She has published more than 30 papers in reputed journals and has two patents. Sher’s lab is focused on cancer translational medicine study to develop promising cancer treatments for clinical application.
Tumor associated antigen L6 (TAL6) is over-expressed in some epithelial cancer cells. We found that more than 80% of breast and lung cancer tissue highly expressed TAL6 as compared to adjacent normal breast tissue. To identify CTL epitopes from TAL6, we synthesized 18 peptides for the HLA-A2 binding assay based on the MHC-binding motif using four computer prediction programs. Positive binders identified by ELISA were immunized in HLA-A2 transgenic (A2 Tg) mice. One peptide induced cytolytic CD8+ T cell responses and inhibit the growth of TAL6-positive tumors (EL4/TAL6/HLA-A2) in A2 Tg mice but not in wild type mice. These results demonstrate that the TAL6-derived CTL epitope could induce HLA-A2-restricted immunity against TAL6-expressing tumor cells. Furthermore, we evaluated human CTL epitope-induced anti-tumor effects in human lung cancer xenograft models. TAL6 derived peptides strongly inhibited tumor growth, cancer metastasis and prolonged survival time in A2 Tg mice immunized with a formulation of T-helper (Th) peptide, synthetic CpG ODN, and adjuvant Montanide ISA-51 (ISA-51). Adoptive transfer of peptide-induced CTL cells from A2 Tg mice into human tumor xenograft SCID mice significantly inhibited tumor growth. The combination of CTL-peptide immunotherapy and gemcitabine additively improved the therapeutic effects. This pre-clinical evaluation model provides a useful platform to develop efficient immunotherapeutic drugs to treat cancer and demonstrates a promising strategy with a benefit of antitumor immune responses worthy of further development in clinical trials.
Gopal Natesan has completed his Doctoral degree (PhD) in Pharmaceutical Chemistry from Hamdard University (Jamia Hamdard) New Delhi, India in 2000 and currently serving as Professor of Medicinal Chemistry & Deputy Dean of Academic Affairs in Faculty of Pharmacy, MAHSA University, Kuala Lumpur, Malaysia. His research focuses on the synthesis of newer small chemical entities, quinazolinones heterocyclic pharmacophore and their preliminary screening in both in-vivo and in-vitro models mainly focusing on pain & inflammation and also for newer microbial agents. He has published >40 articles in indexed journals and presented >80 papers in conferences and received number of honors, recently received “Young Scientist Award” in 2013 by International Society of Nature & Health Care Inc, USA and University of Colombo & University of Kelaniya, Sri Lanka. He was invited speaker at international scientific meetings and conferences and serves as reviewer for several scientific international journals and also as Editorial/Advisory board of various journals.
Pain and inflammation are the main two intolerable health issues faced by healthcare professionals, but the use of modern medicine has associated with unwanted side effects like gastrointestinal bleeding, nephrotoxicity, and cardiovascular adverse effects. The side effects have prompted the discovery of novel analgesic alternatives from the natural sources. Rhizophora genus has been reported for its wide range of therapeutics uses; however there are limited scientific data on its therapeutic applications of Rhizophora stylosa. An attempt has been made to study the analgesic property of Rhizophora stylosa leaves. In this present investigation, different organic extracts of R.stylosa leaves were prepared and evaluated for their analgesic activity by acetic acid induced writhing method and tail immersion method. The powder of the R.stylos’s leaf was extracted using organic solvent in order of increasing polarity i.e.: petroleum ether, chloroform, and methanol by hot percolation method using soxhlet apparatus. The crude extracts were subjected to the biological evaluation at two different doses [100 mg/kg and 250 mg/kg (bw)] in animal model using Swiss Albino mice. All the data was statistically analysed by using one way ANOVA followed Tukey test. From the study, it has been observed that chloroform extracts exhibited good analgesic activity in comparison to other extracts. It has been concluded that the different organic extract of Rhizophora stylosa promised peripheral analgesic (P<0.05) effect with dose dependent manner except methanol extract and all the organic extracts were devoid of central analgesic activity.