Narhari Das
University of Dhaka, Bangladesh
Title: Phytochemical screening and in vitro anthelmintic activity of methanol extract of Terminalia citrina leaves
Biography
Biography: Narhari Das
Abstract
Objective: To evaluate acute and sub-acute toxicity of methanol extract of Terminalia citrina leaves belonging to Combretaceae family in Sprague Dawley rats. Methods: The acute toxicity studies were conducted where the limit test dose of 3200 mg/kg body weight used. Observations were made and recorded systemically on 1, 2, 4, 24 and 48 h after dose administration for behavior, breathing, cutaneous effects, sensory nervous system response or gastrointestinal effects. For the sub-acute toxicity, four groups of 10 female rats were received; distilled water (control), 250, 500 and 1000 mg/kg of extracts respectively every 24 h orally for 28 days. Results: No significant variation in the body and organ weights between the control and the treated group was observed after 28 days of treatment. Haematological analysis and biochemical parameters revealed no toxic effects of the extract. Pathologically, neither gross abnormalities nor histopathological changes were observed. No mortality was recorded in 28 days. Conclusions: It was safer and non toxic to rats even at higher doses and therefore could be well considered for further investigation for its medicinal and therapeutic efficacy.