Neeraj Mishra
ISF College of Pharmacy, India
Title: Glycyrrhetinic acid conjugated embelin loaded PLGA nanoparticles for management of alcohol induced hepatotoxicity
Biography
Biography: Neeraj Mishra
Abstract
The liver diseases such as viral hepatitis, liver cirrhosis,and hepatotoxicity need immediate attention to sustain life and as a result are often exposed to the prolonged treatment with drugs/herbal medications. The ligand decorated nanoparticles play important roles to the specific target drug delivery to the liver sites. Glycyrrhetinic acid (GA) conjugated to the PLGA for effective liver targeting.Preparation of surface modified Embelin loaded GA-PEG-PLGA nanoparticles for specific drug delivery. Surface modified Embelin loaded GA-PEG-PLGA NPs were prepared using nanoprecipitation method and conjugation of GA-PEG-PLGA was attained through hemisuccinate chemistry. These nanoparticles were evaluated by NMR, FTIR, TEM techniques and in vitro release tudies. These surface modified nanoparticles were further evaluated for its targeting mechanism for uptake in liver cells line (Hep G2), liver enzymes and biodistribution studies in liver respectively. The biodistribution of the nanoparticles was assessed by High-performance liquid chromatography (HPLC), and the cellular uptake study was evaluated using Hep G2 cells (liver cells lines). The hepatoprotecttive effect of the Embelin-loaded nanoparticles (GA-PEG-PLGA) was also investigated in-vitro and in-vivo. The surface modified Embelin loaded GA-PEG-PLGA nanoparticles significantly increases the uptake of drug in liver by 2.5 folds more than plain drug. So it can be attributed that Glycyrrhetinic acid has the ligand properties for the receptors presents in the liver, so can be used for liver targeting, as well as it is efficient against hepatotoxicity.