6^th Asia-Pacific Pharma Congress

Biography

Biography: Elisabeth Zeukoo Menkem

Abstract

Uvariodendron calophyllum is a plant of the Annonaceae family used in the treatment of microbial infections in the Centre region of Cameroon. However, little scientific evidence exists in literature on the safety of this plant. The present study was carried out to evaluate the safety of the ethanolic leaf extract of Uvariodendron Calophyllum by the acute and subacute toxicity.The acute and sub-acute toxicity study was conducted in 8 weeks old mice by using OECD 425 and OECD 407 guidelines respectively. In the acute toxicity study, mice were administered a single dose of 2000 mg/kg orally and then observed individually for the first four hours, then over a period of 24 hours and at least once daily for 14 days. In the subacute toxicity studies, ethanolic leaf extract of Uvariodendron Calophyllum (UCL EtoH) was given orally at doses of 100 mg/kg, 200 mg/kg and 400 mg/kg body weight daily for 28 days to male and female mice respectively. General behaviour, adverse effects and mortality were observed throughout the experimental period. Food intake, water intake, body weight, organ weight, hematological and biochemical parameters, histopathological changes were evaluated.The results of acute toxicity of UCL EtoH showed LD50 value >2000 mg/kg by oral route and did not cause any mortality or signs of acute toxicity in the mice tested during the observation period. The subacute toxicity of the UCL EtoH extract revealed no major adverse reactions on the organs and the hematological parameters at the treatment doses of 100 mg/kg, 200 mg/kg and 400 mg/kg. The oral lethal dose of ethanolic leaf extract of UCL is >2000 mg/kg and no observed-adverse-effect level (NOAEL) of the extract for both male and female mice at 400 mg/kg per day for 28 days.